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Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F.A., Hsu, P.D., Konermann, S., Shehata, S.I., Dohmae, N., Ishitani, R., Zhang, F., Nureki, O.

Cell(Cambridge,Mass.) 156: 935-949 (2014)

PDB ID: 4OO8

http://www.rcsb.org/pdb/explore.do?structureId=4oo8

Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA

DOI: 10.2210/pdb4oo8/pdb

Classification: Hydrolase / DNA / RNA

Deposited: 2014-01-31 Released: 2014-02-26

Deposition author(s): Nishimasu, H., Ishitani, R., Nureki, O.

Organism: Streptococcus pyogenes

Expression System: Escherichia coli

Mutation(s): 4

Structural Biology Knowledgebase: 4OO8 (1 model >17 annotations) SBKB.org

Method: X-RAY DIFFRACTION

Resolution: 2.5 Å

R-Value Free: 0.254

R-Value Work: 0.222

Publication

Crystal structure of Cas9 in complex with guide RNA and target DNA

Nishimasu, H., Ran, F.A., Hsu, P.D., Konermann, S., Shehata, S.I., Dohmae, N., Ishitani, R., Zhang, F., Nureki, O.

Cell(Cambridge,Mass.) 156: 935-949 (2014)

PubMed: 24529477 Search on PubMed PubMedCentral: PMC4139937 DOI: 10.1016/j.cell.2014.02.001