Metabolic enzyme beta-galactosidase at high resolution
Cryo-electron microscopy (3DEM) data into Sketchfab
(EMD-2984) was downloaded from the EMDataBank and imported into Chimera. EMD-2984 and PDB 5A1A were exported from Chimera in .obj format. In 3DS Max, the surface corresponding to each beta galactosidase subunit was selected and assigned a color channel. The model was exported from 3DS Max in either .obj or .FBX formats and uploaded to Sketchfab.
Once the model was imported into SF, lighting and background color were adjusted. The SF generated embed code was copied and pasted onto this page (see Fig. 1). Click the “Play” button to start the interactivity.
Both the Cryo-EM surface (EMD 2984) and PDB 5A1A (ribbon formation) were co-exported from 3DS Max and uploaded to Sketchfab. The surface opacity was set to 30%, revealing PDB 5A1A underneath the surface (see Fig. 2).
Cryo-electron microscopy (3DEM) is a field of structural biology that deals with the determination of 3D structures of macromolecular complexes and cells. This emerging field helps to bridges the gap between cell biology and crystallography/NMR.
EMDataBank is the unified global portal for the deposition and retrieval of 3DEM density maps, atomic models and associated metadata.
New advances in electron microscopy reveal molecular structures at resolutions useful for drug discovery.
Produced by Science and the National Cancer Institute.
Animation Credit: Veronica Falconieri and Sriram Subramaniam/LCB/CCR/NCI/NIH
Link to article: http://scim.ag/1dYcNi0
Related studies regarding this structure:
The four protomers of β-galactosidase are indicated on the interactive 3D model above (click on the “annotation” button). Protomers are usually arranged in cyclical fashion to form closed point group symmetries. (See: Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy, Fig. 2)