2.8 Angstrom resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy.

EMDB EMD-6287  primary research article cited below:

2.8 Angstrom resolution reconstruction of the T20S proteasome

Primary publication:
2.8 Angstrom resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy.

Campbell MG, Veesler D, Cheng A, Potter CS, Carragher B
eLife4 e06380-e06380 (2015)
Figure 1.  EMDB EMD-6287  Imported into Sketchfab

Abstract:

Recent developments in detector hardware and image-processing software have revolutionized single particle cryo-electron microscopy (cryoEM) and led to a wave of near-atomic resolution (typically ∼3.3 Å) reconstructions. Reaching resolutions higher than 3 Å is a prerequisite for structure-based drug design and for cryoEM to become widely interesting to pharmaceutical industries. We report here the structure of the 700 kDa Thermoplasma acidophilum 20S proteasome (T20S), determined at 2.8 Å resolution by single-particle cryoEM. The quality of the reconstruction enables identifying the rotameric conformation adopted by some amino-acid side chains (rotamers) and resolving ordered water molecules, in agreement with the expectations for crystal structures at similar resolutions. The results described in this manuscript demonstrate that single particle cryoEM is capable of competing with X-ray crystallography for determination of protein structures of suitable quality for rational drug design.

DOI: http://dx.doi.org/10.7554/eLife.06380.001

Importing Cryo-electron microscopy (3DEM) data into  Sketchfab

(EMD-6287) was downloaded from the EMDataBank and imported into Sketchfab (SF) using standard methods.   The model components were colored using a volume gradient.

Once the model was imported into SF, lighting and background color were adjusted.  The SF generated embed code was copied and pasted onto this page (see Fig. 1).  Click the “Play” button to start the interactivity.

Use the arrows located on the bottom of the viewer ( arows) to navigate to the various positions!

General Information:

Cryo-electron microscopy (3DEM) is a field of structural biology that deals with the determination of 3D structures of macromolecular complexes and cells.   This emerging field helps to bridges the gap between cell biology and crystallography/NMR.
EMDataBank is the unified global portal for the deposition and retrieval of 3DEM density maps, atomic models and associated metadata.
In order to test if this type of data would import into Sketchfab (SF), various structures were downloaded from the above site.  After conversion into acceptable 3d formats, the models were uploaded to SF and embedded on the page above.

 

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